Research Background: EI refers to the capacity to identify, understand, and regulate emotions in oneself and others. Educational medium refers to the language or mode of communication used to deliver educational content within academic institutions. Anxiety is a type of performance-related anxiety that can negatively influence cognitive functioning, learning efficiency, and overall academic outcomes. Emotional Intelligence, academic anxiety, and educational medium are key factors influencing students’ learning and performance. That way in this study, find out the impact of emotional intelligence and educational medium on academic anxiety of the adolescents. Objectives: The study objective was to find out the effect of emotional intelligence on academic anxiety of adolescents. Another objective was to examine the effect of educational medium on academic anxiety of adolescents. Further, the objective was to study the significant interaction effect between emotional intelligence and educational medium on the academic anxiety of adolescents. Procedure: The study included 200 adolescents, with 100 having high emotional intelligence and 100 having low emotional intelligence. Each group was evenly split between English and Marathi medium schools. In the study, the purposive sampling technique was used for the selection of the samples. Emotional intelligence and educational medium were independent variables, while academic anxiety was a dependent variable.The study emotional intelligence scale developed by A. K. Singh and Shruti Narain, and academic anxiety scale developed by Dr. M. Abid Siddiqui and Dr. Atieq Rehman has been used. Conclusions: It can be concluded that adolescents with low emotional intelligence exhibit higher levels of academic anxiety compared to those with high emotional intelligence. Adolescents from English-medium and Marathi-medium schools show similar levels of academic anxiety. Additionally, no significant interaction effect was found between emotional intelligence and educational medium on the academic anxiety of adolescents.