Lipid nanoparticles (LNPs) can enhance the efficacy of mRNA-based cancer therapy by increasing bioavailability upon preserving mRNA integrity and stability until they reach their intended targets. However, the success and safety of LNPs is largely dependent on their intracellular trafficking. Confocal Raman microscopy (CRM) offers insights into the unique spectral fingerprint of samples with subcellular resolution and can shed light on the cell uptake and transport of LNPs. In this preliminary study, we investigate the potential of this imaging modality to track LNPs in live cancer cells.