This study investigated multi- molecular and trait responses of natural populations of the intertidal goby Pomatoschistus microps to seasonal changes, distributed along a latitudinal cline. Fish, as well as environmental data were collected in situ from three locations with different climatic regimes along a north-south gradient in the North Atlantic Ocean during spring and summer seasons. Primary metabolomes and stress biomarkers were analysed in muscle tissue from immature, male and female individuals, together with a qualitative assessment of histopathological traits and fish size. Keywords: macrophysiology, geographical cline, seasonality, metabolomics, phenotypic traits, goby fish, inter-population plasticity