Worldwide, the e-learning has a growth, but failures exist. Previous researches showed that users satisfaction is one of the most important factors in assessing the success of e-learning system implementation and a variety of factors affecting users' satisfaction. In this research, eight independent variables namely learner Internet self-efficacy, instructor response timeliness, e-learning course flexibility, e-learning course quality, technology quality, system usefulness, system ease of use, and diversity in assessment with nine hypotheses were discussed. The Spearman's correlation analysis was used to test initial eight hypotheses and a stepwise multiple linear regression model was conducted to test the ninth hypothesis. The results revealed that e-learning course flexibility, e-learning course quality, technology quality, and diversity in assessment were the critical factors affecting e-learner satisfaction that could not be neglected when implementing successful e-learning courses.