Documentation of the cross-domain adoption of the EuroScienceGateway (ESG) project, showcasing how Galaxy was used and extended to meet the data analysis needs of researchers across biodiversity, climate science, astrophysics, materials science, and biomedical domains. This record outlines ESG’s impact on the onboarding of diverse scientific communities, enabling scalable, reproducible, and FAIR-compliant workflows. Through targeted outreach, infrastructure integration, and community-driven tool development, the project successfully onboarded new user groups and demonstrated Galaxy’s adaptability across multiple scientific verticals. Over 800 tools were integrated into Galaxy during the past 3 years, and dozens of reusable workflows were published to support sensitive data handling, high-throughput image analysis, simulation environments, and federated compute. The deliverable documents use cases, domain-specific onboarding models, training efforts, and collaborative success stories, including the development of the Galaxy Codex and strategic alignment with EOSC, ELIXIR, and NFDI initiatives. Project: EuroScienceGateway was funded by the European Union’s Horizon Europe programme (HORIZON-INFRA-2021-EOSC-01) under grant agreement number 101057388. Document: D5.2 Publication of the usage of EuroScienceGateway by multiple communities Work Package: Work Package 5. Community engagement, adoption and onboarding Tasks: Task 5.1 Biodiversity and Climate Science Task 5.2 Materials Science Task 5.3 Astrophysics Task 5.4 Mentoring and onboarding new communities Lead Beneficiary: University of Oslo (UiO)Contributing Beneficiaries: UiO, ALU-FR, CNRS, UNIFI, UKRI, EPFL, UP, BSC