The Responsible Artificial Intelligence for Digital Cooperation in the Mediterranean Higher Education paper offers a comprehensive, multi-level analysis of how Artificial Intelligence (AI) is reshaping higher education (HE) across the Mediterranean region. It bridges the gap between the recommendations of international organisations, such as UNESCO and the United Nations, national AI strategies, and emerging institutional practices. It calls for coordinated, ethical, open, and inclusive AI integration in HE systems to ensure that digital cooperation and transformation are aligned with societal values, educational equity, and sustainable development. From a policy perspective, it highlights the importance of aligning national AE strategies informed by international frameworks, to ensure that national actions are both globally coherent and locally effective with the operational realities and innovation capacities of HE institutions, promoting human capital development, ethical governance, and sectoral innovation. Noting that these strategies often lack clear implementation pathways within the HE sector, this paper presents a series of recommendations for the creation of national AI–HE coordination mechanisms, the adoption of institutional AI charters, and strategic investment in digital public goods and digital public infrastructure. On the capacity-building front, it identifies a growing momentum within HE institutions to foster AI literacy, pedagogical innovation, and inclusive digital ecosystems. Case studies from across the region illustrate how universities are embedding AI into curricula, training educators, and promoting interdisciplinary research. Algeria’s University 4.0 transition, among others, demonstrates scalable models for institutional transformation. The paper calls for structured capacity-building programmes targeting both students and educators, including micro-credentials, continuous professional development, and support for communities of practice. These efforts must be reinforced by policy environments that recognise HEIs as pivotal actors in national and regional digital transformation. The paper key findings include: AI is a catalyst for institutional transformation, with countries like Algeria, Egypt, and Italy embedding AI into national education strategies. Capacity building is a regional priority, focusing on AI literacy for students, educators, and researchers. Ethical AI use and data governance are central to policy development, with frameworks like the PIO Model and UNESCO guidelines guiding implementation. Digital cooperation is expanding, with initiatives supporting multilingual learning, virtual mobility, and cross-border curriculum development. Crucially, this paper positions HE as a strategic interface between international digital cooperation objectives and national innovation systems, advocating for the co-creation of a Mediterranean Digital Education Charter to harmonise ethical principles, data governance standards, and the use of open practices, including Open Data and Open Educational Resources (OER) across borders. By linking global commitments, national priorities, and institutional practices, it outlines a roadmap for an open, responsible, inclusive, and future-oriented AI transformation in Mediterranean HE. Note from the editors: ** Please note that in case study 5: AI-Powered Pedagogy: Measuring its Impact on Learning Outcomes - Case study of Algeria” authored by Ms Samah Ouatah Feriel, those acknowledged as contributors are not considered to be co-authors and should not be listed as such. The author kindly acknowledges their supervision and guidance in the dissertation where the case derives from and or their support in editing the aforementioned case study **