Ecological concernment and energy extremity of the planet has led to the quest of feasible alternatives to the non-renewable fuel source, FAME (Fatty Acid Methyl Ester) is biodegradable, ecological, alternative, and safe, environmental friendly which has a high flash point and is also termed as Bio-Diesel. In upcoming years, in most of the regions of the world production and application of biodiesel has extrinsic fame. It is usually produced by the method trans-esterification. In this experiment, biodiesel from Calophyllum Inophyllum oil, Karanja Oil Methyl Ester and Cottonseed Oil has been produced using the trans-esterification process. Engine trail have been executed in water cooled, 4- stroke diesel engine. Investigational analysis has been conducted to study the performance and emission on different biodiesel blends of Cottonseed Oil, Karanja Oil Methyl Ester and Calophyllum Inophyllum oil for unequal injection pressures. From the evaluation of obtained results, it can be deduced that the engine operation process is considerably become better with noteworthy subdual in emissions of the CO and HC. https://www.ijiert.org/paper-details?paper_id=140476