This study aims to develop the Student Labor Skills Worksheet and implementing to high school students in Ternate City. Development of Laboratory Skills Worksheet are adapted from four development stages (4-D): define, design, develop, and disseminate. While the Implementation of Laboratory Skills Worksheet aims to gather data about the student laboratory\\\'s initial skills ofhigh school students in Ternate City for science major in grade X and XI. The samples selected by purposive stratified random sampling. The result of analysis showed that the average of initial skill laboratory at school: (1) A accreditation was 57.72 (medium), (2) B accreditation was 56.50 (medium), (3) C accreditation was 21.39 (low). The average of initial skills laboratory of students was 45.20 in medium category.