Alignements from the paper "Life history traits impact the nuclear rate of substitution but not the mitochondrial rate in isopods" submitted to Molecular Biology and Evolution. File "concatenation_382NuclearGenes.fas" corresponds to the concatenation of 382 nuclear genes for 26 Isopod species. File "concatenation_12MtGenes.fas" corresponds to the concatenation of 12 mitochondrial protein coding genes (cytb, cox1, cox2, cox3, atp6, nad1, nad2, nad3, nad4, nad4l, nad5, nad6 ). Genes were aligned with PRANK (Löytynoja and Goldman, 2008) and sites ambiguously aligned were removed with Gblocks (Castresana, 2000). File "Opsine1_cds.fas" corresponds to the alignement of the coding part of the Opsin 1 gene for 26 Isopod species. Intron has been removed as well as non-sens mutations. 26 Mitochondrial genomes: we reconstructed mitochondrial genomes using both the de novo transcriptome assemblies from Francois et al. (2016) and low coverage genome sequencing reads available for 22 species from Lef ebure et al. (2017). Since mitochondrial genomes are present in multiple copies per cell, a very low sequencing effort is sufficient to yield a high coverage for mitochondrial sequences. Using the DNA-seq reads, mitogenomes were assembled with MITObim (Hahn et al. 2013) using the COI gene as a seed to build the complete mitogenome. All annotated genome have been deposited in the ENA project PRJEB14193.