Background: Donor retention is a significant challenge for blood donation centers, and donor education has emerged as a key strategy to improve retention rates. This study explores how educational initiatives influence donor retention from the perspectives of both donors and donation center staff. Objective: To examine how donor education impacts donor retention and identify barriers to delivering effective educational programs. Methods: A qualitative study was conducted using semi-structured interviews with 20 blood donors and 10 donation center staff at a tertiary hospital. Thematic analysis was employed to identify key themes. Results: Donor education reduced anxiety, increased confidence, and encouraged repeat donations, particularly through personalized and post-donation follow-ups. However, barriers such as information overload and resource limitations at donation centers hindered the effectiveness of educational efforts. Conclusion: Simplifying educational materials, personalizing donor engagement, and using digital tools for follow-up can enhance donor retention and improve blood donation programs.