Fishmeal is recognised as a premium protein source in aquaculture diets. However, the highreliance on it, as the main protein source in aquafeeds, has led to over-exploitation of marineresources, resulting in sustainability and economic issues. Efforts to reduce unsustainablecatches have resulted in a reduced supply of marine ingredients for aquafeed production. Inaddition, several plant ingredients have been investigated as alternatives to fishmeal, withcontroversial results. A new potential source that does not compete with human food suppliesand has no economic value, are invasive species such as Lagocephalus sceleratus (Gmelin,1789). These have rapidly established themselves in the Eastern Mediterranean, with variousimpacts on the ecosystem and human welfare, and may provide an alternative marine proteinsource to conventional fishmeal. The aim of the current study was to evaluate in vitro thefishmeal produced by Lagocephalus sceleratus compared to conventional fishmeal.