Colletotrichum juglandium Y. X. Li & X. L. Fan sp. nov. Fig. 7 Etymology. Named after the host genus on which it was collected, Juglans regia L. Typification. China. Sichuan Province, Guangyuan City, Chaotian District, Mianguang Expressway, 32 ° 40 ' 50 " N, 105 ° 59 ' 19 " E, from leaf of Juglans regia L., 10 Oct. 2023, Y. X. Li, L. Lin & X. L. Fan (holotype BJFC - S 2243, ex-holotype culture CFCC 59974). Description. Sexual morph not observed. Asexual morph on OA. Conidiomata acervular, color ranged from peach to light brown. Appressoria and Setae not observed on OA. Conidiophores hyaline, unbranched, approximately cylindrical, 16.0–27.6 × 2.2–4.7, mean ± SD = 20.1 ± 3.2 × 3.2 ± 0.6 µm, n = 30. Conidiogenous cells transparent, cylindrical, formed at the end or side of the hyphae. Conidia straight, hyaline, cylindrical, obtuse at the base, rounded at the apex, with smooth walls and granular contents, 13.2–22.4 × 4.4–6.3 µm, mean ± SD = 16.8 ± 1.8 × 5.4 ± 0.4 µm, L / W radio = 3.1, n = 50. Culture characteristics. Colonies on OA initially white, rapidly growing to 5 cm after 3 d at 25 ° C, and completely covering a Petri dish after 7 days. The aerial mycelium white or gray, with a flocculent cotton like, edge white, center mouse grey. Additional material examined. China. Sichuan Province, Guangyuan City, Chaotian District, Mianguang Expressway, 32 ° 40 ' 36 " N, 105 ° 59 ' 26 " E, from leaf of Juglans regia L., 10 Oct. 2023, Y. X. Li, L. Lin & X. L. Fan (BJFC - S 2245, living culture CFCC 70165). Notes. Colletotrichum juglandium form a solitary clade the C. gloeosporioides species complex (Fig. 3). Colletotrichum juglandium is closely related to C. citrulli, C. gloeosporioides, C. juglandicola and C. peakense (Zhang et al. 2023 a). Sequence identity comparisons reveal that C. juglandium CFCC 59974 differs from other species at various loci: C. citrulli CGMCC 3.20769 (3 / 544 in ITS, 0 / 244 in act, 0 / 228 in chs 1, 10 / 297 in gapdh, and 0 / 324 in tub 2), C. gloeosporioides IMI 356878 (2 / 544 in ITS, 0 / 289 in act, 0 / 236 in chs 1, 18 / 341 in gapdh, and 0 / 324 in tub 2), C. juglandicola CGMCC 3.24312 (2 / 544 in ITS, 0 / 279 in act, 1 / 249 in chs 1, 18 / 341 in gapdh, and 1 / 324 in tub 2), and C. peakense CGMCC 3.24308 (2 / 544 in ITS, 0 / 279 in act, 1 / 249 in chs 1, 13 / 341 in gapdh, and 1 / 324 in tub 2) (Guo et al. 2022; Yu et al. 2022; Zhang et al. 2023 a). Morphologically, C. juglandium differs from C. gloeosporioides by having narrower conidia (L / W ratio: 3.1 vs. 2.6) and differs from C. juglandicola and C. peakense by having wider conidia (L / W ratio: 3.1 vs. 3.3).

Published as part of Li, Yixuan, Lin, Lu, Cao, Jing, Gan, Mingxu & Fan, Xinlei, 2024, Three new species of Colletotrichum (Glomerellales, Glomerellaceae) associated with walnut (Juglans regia) anthracnose from China, pp. 147-167 in MycoKeys 108 on pages 147-167, DOI: 10.3897/mycokeys.108.125382