AbstractIn order to compare the effectiveness of diurnal and nocturnal pollinators, we studied the reproductive biology and pollinators ofTillandsia heterophyllaE. Morren, an epiphytic tank bromeliad endemic to southeastern Mexico. Since anthesis inT. heterophyllais predominantly nocturnal but lasts until the following day, we hypothesised that this bromeliad would receive visits from both diurnal and nocturnal visitors, but that nocturnal visitors would be the most effective pollinators, since they arrive first to the receptive flower, and that bats would be the most frequent nocturnal visitors, given the characteristics of the nectar. Flowering ofT. heterophyllabegan in May and lasted until July. The species is fully self‐compatible, with an anthesis that lasts forca. 15–16 h. Mean volume of nectar produced per flower was 82.21 μl, with a mean sugar concentration of 6.33%. The highest volume and concentration of nectar were found at 20:00 h, with a subsequent decline in both to almost zero over the following 12‐h period.T. heterophyllahas a generalist pollination system, since at least four different morphospecies of visitors pollinate its flowers: bats, moths, hummingbirds and bees. Most of the pollinating visits corresponded to bats and took place in the early evening, when stigma receptivity had already begun; making bats the probable pollinator on most occasions. However, diurnal pollinators may be important as a ‘fail‐safe’ system by which to guarantee the pollination ofT. heterophylla.