Abstract We report the first Quaternary record of the big-eyed bat Chiroderma villosum from South America based on a left mandible fragment collected in the Gruta dos Brejões, late Quaternary of northeastern Brazil. This material has been identified over the course of our revisionary work of the genus Chiroderma, including all species distributed in South America (Chiroderma doriae, Chiroderma salvini, Chiroderma trinitatum, Chiroderma villosum and Chiroderma vizottoi). Our results revealed that several characters of the second lower molar (m2), and of the posterior mandible may be used to identify and to diagnose the South American Chiroderma. We also revisited the historical interpretation of homologies of the lower molar cusps of Chiroderma, and propose a revised molar cusp terminology.