Context. Extensive clearing and modification of habitat is likely to change many facets of the environment including climate and regional food resources. Such changes may result in changes in behaviour in highly mobile fauna, such as flying foxes. Aims.The availability of fruit resources was examined to determine whether grey-headed flying foxes (Pteropus poliocephalus) have feeding preferences related to habitat or dietary items, and whether human usage of the land around the colony site has affected the resources available. Methods. Fruit availability around a colony was monitored from December 2004 to March 2005. Night surveys and faecal analyses were undertaken to determine the distribution of feeding locations, the food species used and the food items consumed by P. poliocephalus. Key results.The amount of food available per hectare in each habitat was similar. However, we found differences in the composition of food trees and the distribution of food resources within each habitat. Ficus species were a major resource with flying foxes observed feeding in figs during every survey and figs identified in droppings over the whole period. Human-modified habitats were used throughout the study period with flying foxes observed in small patches of vegetation and in individual trees without any nearby vegetation. Conclusions. The need for maintaining vegetation, particularly Ficus species, in all areas where flying foxes are found, especially in human-modified habitats and rainforest remnants, is highlighted as this vegetation is of great importance to flying foxes. Other wildlife, such as birds and possums, may also benefit from the maintenance of this vegetation. Implications. Through management of urban resources there is the potential to prevent future conflict situations arising between humans and wildlife, such as can be seen when flying fox colonies are in close proximity to houses.