The Excel file contains data from fixed velocity fatigue experiments for five small-sized Cypriniformes fish species. The recorded data includes common and scientific names of fish species, date and time of test trial, test flume length [cm], flow velocity treatment [cm/s], time-to-fatigue [sec], test water temperature [°C], fish mass [g], fish fork length [cm], fish width [cm], and fish height [cm]. The readme text file explains the column names used in the Excel file. The Rscript file contains the code used to analyse the data.