{"references": ["Beheshti, N., & Mcintosh, A. C. (2007). The bombardier beetle and its use of a pressure relief valve system to deliver a periodic pulsed spray. Bioinspiration and Biomimetics, 2(4), 57.", "Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2), 248-254.", "Cranston, P. S., Hardy, N. B., Morse, G. E., Puslednik, L., & Mccluen, S. R. (2010). When molecules and morphology concur: the 'Gondwanan'midges (Diptera: Chironomidae). Systematic Entomology, 35(4), 636-648.", "Eisner, T., Aneshansley, D., del Campo, M. L., Eisner, M., Frank, J. H., & Deyrup, M. (2006). Effect of bombardier beetle spray on a wolf spider: repellency and leg autotomy. Chemoecology, 16(4), 185-189.", "Eisner, T., & Aneshansley, D. J. (1999). Spray aiming in the bombardier beetle: photographic evidence. Proceedings of the National Academy of Sciences, 96(17), 9705-9709.", "Eisner, T., Eisner, M., Aneshansley, D. J., Wu, C.L., & Meinwald, J. (2000). Chemical defense of the mint plant, Teucrium marum (Labiatae). Chemoecology, 10(4), 211-216.", "Gullan, P., & Cranston, P. (2010). Insect systematics: phylogeny and classification. The Insects: An Outline of Entomology. Fourth ed. Wiley-Blackwell, Oxford, 189-222.", "Harborne, J. B. (2014). Introduction to Ecological Biochemistry, Academic press,4th Edition, 1-384", "Holldobler, B., & Wilson, E. O. (1990). The ants, Belknap Press of Harvard University Press, Cambridge, MA, 1-732.", "Moore, B., & Brown, W. (1979). Chemical composition of the defensive secretion in Dyschirius bonelli (Coleoptera: Carabidae: Scarittnae) and its taxonomic significance. Austral Entomology, 18(2), 123-125.", "Rice, S. D., Short, J. W., Carls, M. G., Moles, A., & Spies R. B. (2007). The Exxon Valdez oil spill. In: Spies, R. B., editor. Long-term Ecological Change in the Northern Gulf of Alaska. Amsterdam Elsevier, 419-520.", "Schnepf, E. (1969). Sekretion und Exkretion bei Pflanzen. Protoplasmatologia, Handbuch der Protoplasma-forschung, Bd. 8, Physiologie des Protoplasmas, Springer, New York.", "Will, K.W., Attygalle, A.B., & Herath, K. (2000). New defensive chemical data for ground beetles (Coleoptera: Carabidae): interpretations in a phylogenetic framework. Biological Journal of the Linnean Society, 71(3), 459-481."]}

The bombardier beetle Pheropsophus hilaris having a pair of defensive organs consisted of many small synthetic lobes, large reservoirs and collecting ducts. The defence secretary lobes of P. hilaris secrete aqueous hydrogen peroxide and hydroquinones, which are stored in the large quantity in the collecting reservoirs. The modern research is oriented towards attaining health and economic benefits of mankind. Now- a- days, the farmers are using large number of pesticides and insecticides to control the pest and insect. The poor knowledge among the farmers is the main reason for pollution. In order to reduce pollution by contaminating the amount of pesticides, biological control is also advocated and attempted on different scale. In recent years, in addition to bio control, bio pesticides came into existence for integrated pest management. In view of this, it has been programmed to study the GC MASS and HPLC analysis of the defence secretion of P. hilaris.