Introduction: In order to evaluate the clinical features, range of microbial flora, antibiotic susceptibility, and develop an empiric antimicrobial therapy, this study was conducted on patients with diabetic foot ulcers (DFU). Method: Between December 2020 and November 2021, clinical information and tissue samples were gathered from 100 diabetic foot ulcer patients. Clinical and microbiological data was evaluated after the samples were handled in accordance with Clinical and Laboratory Standards Institute recommendations. Results: Males in their fourth and fifth decades of life had the highest prevalence of DFU in this sample of 100 patients. The majority of patients experienced neuropathy and poor glycemic control. Gram-negative bacteria made up 73.6% of the 105 bacterial isolates isolated from 96 samples, while Gram-positive bacteria made up 27.2%. The majority of samples (48.5%) revealed the growth of a single bacterium, followed by the growth of two bacteria and polymicrobial growth in 28.5% and 15.1%, respectively, of tissue samples. The most common isolation (27.2%) was Pseudomonas, which is responsive to imipenem (91%), amikacin (86.5%), gentamicin (83.2%), and cefotaxime (81%). Staphylococcus aureus (19.0%) is the next most common isolate, and it is sensitive to amikacin and gentamicin (99%), as well as ofloxacin (91%). Ampicillin, amoxicillin-clavulanic acid, Proteus, and Klebsiella were all very resistant to these medications. Conclusion: This study demonstrated that DFU are typical in the fourth and fifth decades of life. Most infectious organisms are gram-negative bacteria. The majority of bacteria, both Gram positive and Gram negative, are sensitive to aminoglycosides and have varying degrees of resistance to routinely used antibiotics. DFU infections can be treated with amikacin and gentamicin as empiric antibiotics.

Introduction: In order to evaluate the clinical features, range of microbial flora, antibiotic susceptibility, and develop an empiric antimicrobial therapy, this study was conducted on patients with diabetic foot ulcers (DFU). Method: Between December 2020 and November 2021, clinical information and tissue samples were gathered from 100 diabetic foot ulcer patients. Clinical and microbiological data was evaluated after the samples were handled in accordance with Clinical and Laboratory Standards Institute recommendations. Results: Males in their fourth and fifth decades of life had the highest prevalence of DFU in this sample of 100 patients. The majority of patients experienced neuropathy and poor glycemic control. Gram-negative bacteria made up 73.6% of the 105 bacterial isolates isolated from 96 samples, while Gram-positive bacteria made up 27.2%. The majority of samples (48.5%) revealed the growth of a single bacterium, followed by the growth of two bacteria and polymicrobial growth in 28.5% and 15.1%, respectively, of tissue samples. The most common isolation (27.2%) was Pseudomonas, which is responsive to imipenem (91%), amikacin (86.5%), gentamicin (83.2%), and cefotaxime (81%). Staphylococcus aureus (19.0%) is the next most common isolate, and it is sensitive to amikacin and gentamicin (99%), as well as ofloxacin (91%). Ampicillin, amoxicillin-clavulanic acid, Proteus, and Klebsiella were all very resistant to these medications. Conclusion: This study demonstrated that DFU are typical in the fourth and fifth decades of life. Most infectious organisms are gram-negative bacteria. The majority of bacteria, both Gram positive and Gram negative, are sensitive to aminoglycosides and have varying degrees of resistance to routinely used antibiotics. DFU infections can be treated with amikacin and gentamicin as empiric antibiotics.