In recent years, the quality and utilization of motor gasoline have become increasingly critical, influencing both technological advancements and economic growth. With the rising demands for enhanced motor gasoline performance, there is a pressing need for innovative types that offer superior characteristics. One of the primary requirements for motor gasoline is optimal knock resistance, ensuring smooth engine operation across various modes. This study explores the rapid assessment of the detonation properties of motor fuels through advanced physico-chemical methods that monitor the chemical reactions and associated physical phenomena of these fuels. A novel approach discussed in this article involves the cold-flame combustion method, utilizing an oxidation reaction with oxygen-enriched gases. This method includes measuring specific physical effects correlated with fuel detonation properties. The introduction of ozone, a potent oxidizing agent and an allotrope of oxygen, presents new opportunities for enhancing the quality control of hydrocarbon fuels. Focusing on product quality improvement and increased efficiency, this research investigates a method that incorporates ozone derived from the ambient air. The development and application of this method could significantly impact the refinement of hydrocarbon fuels, making the study of such innovative techniques pertinent and timely.