Database of protein sequences obtained using ProHap (https://github.com/ProGenNo/ProHap) on the data set of phased genotypes published by the 1000 Genomes Project, aligned with the GRCh38 genome build (https://www.internationalgenome.org/data-portal/data-collection/grch38). We used Ensembl v.110 for the mapping of coordinates between genes, exons, and transcripts. This data set contains six compressed directories, five representing the superpopulations included in the 1000 Genomes Project (https://catalog.coriell.org/1/NHGRI/Collections/1000-Genomes-Project-Collection/1000-Genomes-Project), and one created using all the samples included in the 1000 Genomes data set: AFR - African AMR - American EUR - European SAS - South Asian EAS - East Asian ALL - all participants in the 1000 Genomes Project Each of the directories contains the following files: F1: The concatenated fasta file ready to be used with search engines, contains the following: Protein haplotype sequences obtained by ProHap, using alleles with at least 1 % frequency within the selected population Reference proteome as per Ensembl v. 110 Contaminant sequences from the cRAP project (https://www.thegpm.org/crap/) F2: Additional information about the haplotype sequences, to be used for mapping identified peptides to the original haplotypes F3: Translations of haplotype cDNA sequences, before merging with the reference proteome For further description of the files, please refer to https://github.com/ProGenNo/ProHap/wiki/Output-files. For the usage of these databases with search engines, and downstream anaylsis of identified peptides, please refer to the project's wiki page: https://github.com/ProGenNo/ProHap/wiki/Using-the-database-for-proteomic-searches.