An attempt has been made to study of Ethnomedicinal plants used by Mising tribe of Dhemaji district, Assam for the treatment of 48 different diseases like Jaundice, Diabetes, Asthma, Dysentery, Muscular pain, Tonsillitis, Uvulitis, Ringworm, Kidney problem, Small pox, Lactation problem etc. Main aim of the study is to document the indigenous knowledge of the Mising tribe. In this study a total of 142 plant species belonging to 63 families were collected. The prominent family was Euphorbiaceae with 7 species and leaf is used majority of cases with 67 species for the treatment of different diseases. Other different used parts are rhizome, flower, fruit, bark, root, whole plant, seed etc.