The objective of this study was to detect and determine the cholesterol level in different animal proteins. Five different animal meat samples, including beef, pork, deer meat, goat meat, and rabbit meat, were used for this study. UV-spectrophotometric analysis and gas-chromatographic analysis (Gas Chromatography Mod Agilent 6890 CA, USA, equipped with an on-column automatic injector) were employed. Oils from the different meats were extracted using a Soxhlet extractor. UV-Spectrophotometric analysis of different animal meat samples revealed that beef had the highest value of cholesterol (17.73 mg/L), while pork had the least value (8.08 mg/L). Gas-chromatographic analysis of saturated fatty acids from different animal meat samples revealed that deer had the highest percentage of saturated fatty acids (85.21%), while pork had the least percentage (68.99%). The gas-chromatographic analysis of unsaturated fatty acids from different animal meat samples revealed that pork had the highest percentage of unsaturated fatty acids (31.01%), while beef had the least percentage (14.55%). From the statistical analysis of variance for saturated fatty acids in different meat samples Since the p-value of 0.11 is greater than 0.05 (p > 0.05), this implies that there is no significant difference in the values of saturated fatty acids in different meat samples.