ABSTRACT Generally, many of the fruits and vegetables skins are thrown in the garbage or fed to livestock. These are having very rich in bioactive components which are considered to have beneficial effects on health. But the aim of this study was to evaluate the antibacterial activity and antifungal activity. Here by we present the antibacterial and antifungal activity of different peels of vegetable and fruits due to their numerous antioxidants and odd compounds. The peel powders were effective in inhibiting antibacterial and antifungal activity. We aimed to evaluate the activity of different vegetable and fruit peels powders against E. coli, Lacto bacillus, Proteus vulgaris, Staphylococcus aureus, and Saccharomyces cerevisiae., were known to causes antibiotic resistant infections. We performed the agar diffusion method and indicated the bactericidal and fungicidal action. We suggest that peels of fruits and vegetables might be effective sources as they have numerous antioxidants. KEYWORDS: Antibacterial activity, Antifungal activity, Peels of different Vegetables and Fruits and different Microorganisms.