Introduction: HIV/AIDS has been acknowledged as an endemic and deadly disease which can spread rapidly. This study proposes to reveal the effect and effectiveness level of e-health literacy on the HIV/AIDS transmission preventive behavior of teen punks. Methods: This study employed a quantitative experimental design that falls under action research. It carried out an accidental sampling approach and was conducted with 82 participants who had signed an informed consent form. Data were collected using a 5-point Likert scale questionnaire. The selected statistical analyses were Paired Sample T-Test and N-Gain score. Both were to determine the effectiveness level with a significance of 95%. Results: This study shows that 80.5% of the participants were male, with an average age of 25.95±6.442. Furthermore, 65.9% were unmarried, and 62.2% had completed high school education. Additionally, gender, age, marital status, and education were found to have no significant influence on preventive behavior. However, support for e-health literacy significantly impacts preventive behavior (p=0.00), with a mean difference of 13.38. Support for e-health literacy effectively reinforces preventive behavior in the context of HIV/AIDS transmission (n-gain score=59.4%). Conclusion: Support for e-health literacy effectively reinforces preventive behavior in the context of HIV/AIDS transmission of punk teenagers.