Solid wastes such as municipal wastes and agricultural wastes can pollute our environment if they are not disposed properly. In contrast, under manipulation, they can be changed into valuable products such as bio-enzymes and bio fuel. Bio-enzymes derived from kitchen wastes and agricultural wastes are cost effective and can be utilized in industry and agriculture with cutting in cost. This research is carried out to provide information about the cost-effective bio-enzymes with emphasis on their effect on germination and growth of seedlings of peanut (Arachis hypogea L. subsp. fastigiata). For bio-enzyme production, kitchen wastes such as onion-peels, cruciferous and citric fruits were used as solid substrate. Fermentation method was solid state fermentation with and without isolated microbes. The fermented formulation was that the mixture of three substrates at a weight/weight/weight ratio of 1:1:1 was placed into four airtight plastic containers containing the mixture of molasses and water at a volume/volume ratio of 1:10. Isolated bacteria, P1, P2 and P105 were introduced into three formulated mixtures, BE2, BE3, and BE4, respectively. All of them were fermented at 25 - 30 for three months. From this research, different hydrolytic activities of these four formulated bio-enzymes were investigated and their concentration being likely to impact on the germination and growth of peanut were examined in comparison to Hydro treatment used as negative control. Among BE1, BE2, BE3, BE4 and Hydro treatments, BE3 treatment at 1:200 dilution significantly showed the most effective activity on germination index, vigor index, branches per plant and pods per plant with 6.31 ± 0.29, 44.9 ± 23.63, 6.85 ± 0.15 branches per plant and 28.98 ± 0.07 pods per plant, respectively. This research shed light on the effect of bio-enzymes on peanut plant cultivation, particularly BE3, and it may become a potential for smallholder farmers.