Maintaining hygiene during menstruation is very important for women. Working women do not always get an adequate environment to practice hygiene. Workers are most deprived in various socio-economic contexts, including health, in the tea plantations of Bangladesh. In the garden area, female workers are considered a vulnerable population due to the lack of an environment to follow menstrual hygiene, inadequate education and knowledge, low income, etc. The study was conducted on the menstrual health and hygiene practices of female workers in the Kodala tea estate area in Rangunia Upazila, Chattogram, Bangladesh. Following a mixed method, the study explored the hygiene practices, work environment, and benefits and challenges faced by female workers during menstruation. The study showed that most of the female workers are indifferent to hygiene practices. Lack of awareness, a poor environment, a poor standard of living, and low wages have been found to have a negative impact on menstrual health and hygiene practices. It is found that 90% of female workers suffer from a variety of health issues as a result of poor menstrual hygiene, such as genital itching, sexual intercourse pain, increased white discharge, etc. 98% of female workers find it difficult to work during menstruation. The study recommended the establishment of proper healthcare facilities to ensure health safety for female workers. Simultaneously, a better standard of living along with regular awareness campaigns can provide a sustainable and healthy work environment for the female workers of the tea estate.