
This report presents a guide to selecting high-quality commercial antibodies against Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 (SHIP1), encoded by INPP5D, by Western blot and immunoprecipitation, using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. Work presented in this report was supported in part by the Accelerating Medicines Partnership Program for Alzheimers Disease (AMD-AD) established by the National Institutes of Aging under award number U54AG065187.
SH2 domain- containing inositol phosphatase 1, antibody characterization, Inositol polyphosphate-5-phosphatase D, p150Ship, INPP5D, Western blot, Inositol polyphosphate-5- phosphatase of 145 kDa, Alzheimer's disease, immunoprecipitation, SHIP1, Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1, Q92835, Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, antibody validation
SH2 domain- containing inositol phosphatase 1, antibody characterization, Inositol polyphosphate-5-phosphatase D, p150Ship, INPP5D, Western blot, Inositol polyphosphate-5- phosphatase of 145 kDa, Alzheimer's disease, immunoprecipitation, SHIP1, Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1, Q92835, Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, antibody validation
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