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The Use Of Acid-Aluminium Tolerant Bradyrhizobium Japonicum Formula For

Authors: Mubarik, Nisa Rachmania; Tedja Imas; Wahyudi, Aris Tri; Triadiati; Suharyanto; Happy Widiastuti;

The Use Of Acid-Aluminium Tolerant Bradyrhizobium Japonicum Formula For

Abstract

{"references": ["P. Somasegaran and H.J. Hoben. Methods in legume-Rhizobium\nTechnology. Paia: University of Hawaii , 1994.", "M.K. Abbasi, A. Majeed, A.Sadiq, and S.R. Khan.. Application of\nBradyrhizobium japonicum and phosphorus fertilization improved\ngrowth, yields and nodulation of soybean in the sub-humid hilly region\nof Azad Jammu and Khasmir, Pakistan. Plant Prod. Sci., vol. 11, pp.\n368-376, 2008.", "A. Ayanaba, S. Asanuma, and D.N. Munns, \"An agar plate method for\nrapid screening of Rhizobium tolerance to acid aluminium stress,\" Soil.\nSci. Am. J ., vol. 47, pp. 256-258, 1983.", "R.M. Atlas and R. Bartha. Microbial Ecology, Fundamentals and\nApplication. 4th Ed. Menlo Park: Addison Wesley Longman, Inc,\n1998.", "J. Denarie, P. Debelle, and C. Rosenberg, \"Signaling and host range\nvariation in nodulation, \" Ann. Rev. Microbiol.,vol. 46, pp.497-531,\n1992.", "T. Endarini, A.T. Wahyudi, Tedja-Imas, \"Selection of indigenous\nBradyrhizobium japonicum tolerant acid-aluminium medium\" (in\nIndonesia language), Hayati, vol. 2, pp. 74-79, 1995.", "H. Habibah. Effectivity of acid-aluminium tolerant Bradyrhizobium\njaponicum on the growth of Slamet [thesis, in Indonesia language].\nBogor: Postgraduate School, Bogor Agricultural University, 2008.", "A.R.F. Situmorang, N.R. Mubarik, and Triadiati, \"The use of inoculant\nacid- aluminium tolerant Bradyrhizobium japonicum for soybean\ngrowth on acid soils,\" Hayati J. Biosci., vol. 16, pp. 157-160, 2009.", "A.T. Wahyudi, A. Suwanto, Tedja-Imas and A. Tjahyoleksono,\n\"Screening of acid-aluminium tolerant Bradyrhizobium japonicum strain\nanalysis of marker genes and competition in planta,\" Aspac. J. Mol. Biol.\nBiotechnol . vol. 6, pp. 13-20, 1998.\n[10] X. Parveen, D.T. Webb, D. Borthakur, \"The symbiotic phenotypes of\nexopoly- saccharide defective mutants of Rhizobium sp. strain TAL1145\ndo not differ on determinate and indeterminate nodulating tree legume,\"\nMicrobiology, vol. 143, pp. 1959-1967, 1997.\n[11] A.H. Lounch, Karen, and J Miller. \"Synthesis of low-molecular-weight\nform of exopoly- saccharide by Bradyrhizobium japonicum USDA\n110,\" Appl. Environ. Microbiol., vol. 2, pp. 1011- 1014, 2001.\n[12] R. Saraswati,\"Technology of multipurpose microbial fertilizer\nsupporting sustainable system of soybean production,\" J Mikrobiol\nIndones, vol. 4, pp. 1-9, 1999."]}

Land with low pH soil spread widely in Indonesia can be used for soybean (Glycine max) cultivation, however the production is low. The use of acid tolerant soybean and acidaluminium tolerant nitrogen-fixing bacteria formula was an alternative way to increase soybean productivity on acid soils. Bradyrhizobium japonicum is one of the nitrogen fixing bacteria which can symbiose with soybean plants through root nodule formation. Most of the nitrogen source required by soybean plants can be provided by this symbiosis. This research was conducted to study the influence of acid-aluminium tolerant B. japonicum strain BJ 11 formula using peat as carrier on growth of Tanggamus and Anjasmoro cultivar soybean planted on acid soil fields (pH 5.0- 5.5). The results showed that the inoculant was able to increase the growth and production of soybean which were grown on fields acid soil at Sukadana (Lampung) and Tanah Laut (South Kalimantan), Indonesia.

Keywords

Bradyrhizobium japonicum, Tanggamus cultivar soybean, acid-aluminium tolerant mutant, acid soils

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