This study, carried out between June 2019 and September 2020 in the localities of Kadiolo, Dioïla and Diéma, aimed to determine the prevalence of Babesia bovis and Babesia bigemina. It involved blood analysis of 648 cattle samples (327 females and 321 males) and tick collection for Babesia bigemina and Babesia bovis parasites and their vectors. During the dry season, 72 cattle tested positive for Babesia bovis out of a total of 324 examined, giving a prevalence rate of 22.22%. On the other hand, during the rainy season, 128 cattle were positive for Babesia bovis out of a total of 324 examined, for a prevalence rate of 39.50%. The study revealed that the vectors of Babesia bigemina and Babesia bovis are more abundant during the rainy season than in the dry season.