Fig. 4. Phylogenetic analysis of CPR family members based on 47 amino acid sequences (28 species). The mid-point root neighbor-joining (NJ) tree was constructed using the MEGA6 program (Saitou and Nei, 1987; Tamura et al., 2013). The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches (Felsenstein, 1985). The evolutionary distances were computed using the JTT matrix-based method (Jones et al., 1992b). The accession number of the CPR amino acid sequence from each species is represented at the end of the scientific name. The CPR isolated from C. stellatopilosus is indicated by a dark circle. The tree was drawn to scale with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree (the scale bar).