Fig. 4 in Sequential oxidation of Jasmonoyl-Phenylalanine and JasmonoylIsoleucine by multiple cytochrome P450 of the CYP94 family through newly identified aldehyde intermediates
Widemann, Emilie; Grausem, Bernard; Renault, Hugues; Pineau, Emmanuelle; Heinrich, Clément; Lugan, Raphaël; Ullmann, Pascaline; +5 Authors
Widemann, Emilie; Grausem, Bernard; Renault, Hugues; Pineau, Emmanuelle; Heinrich, Clément; Lugan, Raphaël; Ullmann, Pascaline; Miesch, Laurence; Aubert, Yann; Miesch, Michel; Heitz, Thierry; Pinot, Franck;
Fig. 4 in Sequential oxidation of Jasmonoyl-Phenylalanine and JasmonoylIsoleucine by multiple cytochrome P450 of the CYP94 family through newly identified aldehyde intermediates
Fig. 4. UPLC-MS/MS analysis of metabolites produced by CYP94B1 and CYP94B3 with JA-Phe. JA-Phe was incubated with microsomes of yeast expressing CYP94B1 in presence (panels A–D) or in absence (panels E–H) of NADPH and with microsomes of yeast expressing CYP94B3 in presence (panels I-L) or in absence (panels M-P) of NADPH. JA-Phe (100 μM) and microsomes (0.4 mg protein) were incubated for 20 min at 27 °C. Residual substrate (JA-Phe) and metabolites (12OH-JA-Phe, 12CHO-JA-Phe and 12COOH-JA-Phe) were identified by LC-MS/MS based on their retention times and detection in multiple reaction monitoring.
Biodiversity, Taxonomy
Biodiversity, Taxonomy