This dataset presents georeferenced measurements collected at the Nivolet Plain in Gran Paradiso National Park (GPNP), western Italian Alps. The dataset includes the Net Ecosystem Exchange (NEE), Ecosystem Respiration (ER) and meteo-climatic variables, i.e. air and soil temperature, air relative humidity, soil volumetric water content, atmospheric pressure and solar irradiance. The measurements were conducted between 2017 and 2023 at five different sites at an elevation of approximately 2550-2750 meters a.s.l. To estimate NEE and ER, we employed the flux chamber method, measuring the temporal variation of carbon dioxide (CO2) concentration inside the chamber over a period of about 90 seconds. We used a customized portable non-steady-state dynamic flux chamber, paired with an InfraRed Gas Analyzer (IRGA) and a portable weather station. Measurements were taken at around 20 points per site during the snow-free season, spanning from June to October. The dataset is provided in a comma-separated text file (.csv) format. Each record corresponds to a single measurement point, with semicolons used as separators. The "NA" notation indicates values that are not available or have been excluded during quality control processes (e.g., due to battery failure). We use point as decimal separator. The sign convention for the fluxes is: a negative value indicates a CO2 flux from the atmosphere to the ecosystem, while a positive value represents a CO2 flux from the soil/ecosystem to the atmosphere. Consequently, ER values are positive, while NEE values can be positive or negative. The units for NEE and ER fluxes are molCO2 m-2 day-1 and μmolCO2 m-2 second-1. The first values in each record of the dataset indicate the observation details (sampling date, site, etc.), followed by the corresponding measured or calculated variables. NEE and ER values were estimated from the slope of the linear regression of CO2 concentration over time (ppm s-1) using a laboratory calibration curve. The calibration curve was created by relating known and pre-set CO2 fluxes (within the range expected in the field) with the corresponding measured slopes. The flux values were then scaled up based on the area of the chamber base (0.036 m2) and adjusted using the ratio of atmospheric pressure and air temperature during the measurement to those recorded during the calibration in the laboratory.