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An updated checklist and a DNA barcode library for the earthworms (Crassiclitellata, Oligochaeta) of Corsica, France

Authors: Marchán, Daniel Fernández; Gérard, Sylvain; Hedde, Mickael; Rougerie, Rodolphe; Decaëns, Thibaud;

An updated checklist and a DNA barcode library for the earthworms (Crassiclitellata, Oligochaeta) of Corsica, France

Abstract

Les études disponibles sur la faune lombricienne de la Corse font état d’un total de 36 espèces, mais cette diversité régionale est probablement sous-estimée si l’on considère les efforts d’échantillonnage relativement modestes réalisés jusqu’à présent. Nous avons mené une campagne d’échantillonnage dans le cadre du programme « La Planète Revisitée », avec comme objectif d’augmenter la couver- ture d’échantillonnage et d’analyser la diversité régionale en combinant l’utilisation des caractères morpho-anatomiques classiques à celle des codes-barres ADN. Nous avons échantillonné un total de 31 sites, ce qui a permis d’identifier 22 espèces précédemment citées et huit nouvelles signalisa- tions appartenant aux familles Acanthodrilidae Claus, 1880, Hormogastridae Michaelsen, 1900 et Lumbricidae Rafinesque-Schmaltz, 1815. Nous avons obtenu 525 codes-barres ADN qui nous ont permis de délimiter 48 lignées génétiques, soulignant l’importance des techniques moléculaires pour inventorier la diversité des vers de terre. Parmi ces lignées, dix sont soutenues par la morphologie et seront décrites comme espèces nouvelles pour la science dans un article de taxonomie à venir. Les affinités biogéographiques des espèces identifiées montrent un taux relativement faible d’espèces cos- mopolites par rapport à d’autres îles méditerranéennes, un degré d’affinité avec les faunes italiennes et sardes, et un taux élevé d’endémisme (qui pourrait être équivalent à celui des îles Baléares et de la Sardaigne). Des analyses phylogéographiques comparatives pourraient aider à comprendre l’histoire de la colonisation et la diversification des faunes de vers de terre des îles méditerranéennes et à com- prendre les facteurs qui ont façonné les différences dans leur diversité.

Available studies of the earthworm fauna of Corsica reported a total of 36 species, but this regional diversity is probably underestimated considering the relatively modest sampling efforts achieved so far. We conducted a sampling campaign in the context of the program “Our Planet Reviewed”, with the aim to increase sampling coverage and to analyze already known regional diversity with a combination of classical morpho-anatomic characters and DNA barcoding. We sampled a total of 31 locations, and identified 22 previously recorded species and eight new citations belonging to the families Acanthodrilidae Claus, 1880, Hormogastridae Michaelsen, 1900 and Lumbricidae Rafinesque- Schmaltz, 1815. We obtained 525 COI barcodes which clustered into 48 genetic lineages, highlight- ing the importance of molecular techniques for earthworm diversity surveys. Of these lineages, ten are supported by multiple lines of evidence and will be described in a forthcoming taxonomy paper as species new to science. Biogeographical affinities of the identified species showed a lower rate of cosmopolitan species compared to other Mediterranean islands, a certain degree of affinity with Italy and Sardinia, and a high rate of endemism (which could be mirrored by the Balearic Islands and Sardinia). Comparative phylogeographic analyses are needed to understand the history of coloniza- tion and diversification of earthworms on Mediterranean islands, and to investigate the drivers that shaped differences in their diversity.

Country
France
Keywords

Annelida, Acanthodrilidae, Mediterranean islands, new records, species list, Biodiversity, Hormogastridae, endemism, [SDV.BID.SPT] Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, Crassiclitellata, Animalia, Clitellata, DNA barcoding, Diporodrilidae, Lumbricidae, Taxonomy

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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