This study aims to determine the effect of motivation on employee performance through organizational culture variables at the Pasuruan City Civil Service Police Unit. This research is an explanatory research (explanatory research) which aims to test the proposed hypothesis about the effect of motivation on employee performance and organizational culture. This study uses quantitative data analysis with path analysis and classical assumption test using non-probability sampling technique, namely saturated sampling (census). The population and sample in this study were all employees at the Pasuruan City Civil Service Police Unit with a total of 104 employees. From the results of this study indicate that (1) motivation has a direct and significant effect on employee performance, (2) motivation has a direct and significant effect on organizational culture, (3) organizational culture has a direct effect on employee performance, and (4) organizational culture can mediate indirectly between motivation and employee performance.