The current work represents summarised data on the bee fauna in Serbia from previous publications, collections, and field data in the period from 1890 to 2020. A total of 706 species from all six of the globally widespread bee families is recorded; of the total number of recorded species, 314 have been confirmed by determination, while 392 species are from published data. Fourteen species, collected in the last three years, are the first published records of these taxa from Serbia: Andrena barbareae (Panzer, 1805), A. clarkella (Kirby, 1802), A. fulvicornis (Schenck, 1853), A. intermedia (Thomson, 1870), A. lapponica (Zetterstedt, 1838), A. pandellei (Pérez, 1895), A. paucisquama (Noskiewicz, 1924), A. simillima (Smith, 1851), Panurginus herzi (Morawitz, 1892), Epeoloides coecutiens (Fabricius, 1775), Nomada leucophthalma (Kirby, 1802), Chelostoma nasutum (Pérez, 1895), Hoplitis claviventris (Thomson, 1872), and Dasypoda pyrotrichia (Förster, 1855). Almost all the species recorded so far in Serbia belong to the West-Palaearctic biogeographical region, except Megachile sculpturalis (Smith, 1853), which is an alien invasive species native to East Asia. According to the European Red List of bees, 221 species listed in this paper were assessed as Data Deficient; threatened species mostly belong to the families Apidae with 13 species, Colletidae with eight species, and Halictidae with five species. This study contributes to the knowledge of the distribution of bee species in Europe. The present work provides a baseline for future research of wild bee diversity in Serbia and neighbouring regions at the local and regional levels, and a basis for their conservation.