Defining phylogenetic relationship of Nepeta x tmolea and its parents via DNA barcoding

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 23 Oct 2019 Turkey Publisher:Pensoft PublishersJournal:PhytoKeys, volume 134, pages 83-96 (issn: 1314-2011, eissn: 1314-2003,

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Authors: Taner Özcan;

doi: 10.3897/phytokeys.134.38238

pmid: 31686955

pmc: PMC6821826

handle: 20.500.12462/10499

Defining phylogenetic relationship of Nepeta x tmolea and its parents via DNA barcoding

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Abstract

Nepeta viscidaand N. nuda subsp. nuda and N. × tmolea were examined in this study. Mainly fresh leaf pieces, dried with silica grains, were used for DNA extraction procedures via DNA isolation kits. Standard PCR techniques were executed using three different primer sets (one nuclear DNA region (nrITS) and two chloroplast DNA regions (rpl32-trnL andtrnA(Leu)-trnA(Phe)-trnL-F). DNA sequences were analysed and evaluated using different molecular approaches and software. Consequently, the inconstant molecular structure and hybrid nature of N. × tmolea specimens were shown and interpreted in this study. According to our result, N. × tmolea have some intermediate characters compared to its parents. nrITS data give more information phylogenetically, and also the most polymorphic loci are seen in nrITS data. Morphological and molecular data contribute to define separation of N. × tmolea. Consequently, the inconstant molecular structure and hybrid nature of N. × tmolea specimens were shown and interpreted in this study.

Country

Turkey

Related Organizations

Balıkesir University
Turkey
Balikesir University
Turkey

Keywords

Lamiaceae, hybrid, Turkey, Botany, Molecular, phylogeny, Hybrid, Lamiales, Tracheophyta, Magnoliopsida, QK1-989, Nepeta, molecular, Plantae, Phylogeny, Research Article

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