Many poikilotherms have the ability to change body color for homeostatic regulation, conspecific communication or predator deterrence. Physiological color change is a rapid, reversible mode of color change regulated by neuromuscular or neuroendocrine system and has been observed in several anuran species. Here we report the occurrence of physiological color change in the tree frog Rhacophorussmaragdinus (Blyth, 1852) (Amphibia, Anura, Rhacophoridae) for the first time from Namdapha Tiger Reserve, Arunachal Pradesh, India. Probable proximate causes of the behavior are discussed along with an overview of physiological color change in species of the family Rhacophoridae and nature of color change observed.