AugochloraSmith, with 127 valid species, is the most widespread genus of Augochlorini bees, ranging from Argentina to southern Canada, including the Caribbean islands. The genus is divided into three subgenera,Augochloras. str.,OxystoglossellaEickwort, and the fossilElectraugochloraEngel. The extant subgenera were traditionally diagnosed by their nesting substrate, social behavior and morphology. However, accumulating evidence suggests that these features are not reliable for their separation, especially with the discovery of an enigmatic species sharing characteristics from both subgenera. Our objective is to provide a phylogenetic hypothesis to evaluate the monophyly of the extant subgenera and to place a new species, Augochlora (Augochlora) intermedia sp. nov. For this purpose, we compiled 110 unordered characters for 40 species ofAugochloraplus seven outgroup species and analyzed under parsimony and Bayesian inference. Topologies were very similar under both frameworks, allowing us to consistently characterize a few major lineages. Our results demonstrate that the extant subgenera correspond to monophyletic groups and the new species is sister group to remainingAugochloras. str. species. Both subgenera are widespread in the Western Hemisphere, with species groups differing in range and distributional patterns. Our interpretation is thatAugochloraarose in South America, subsequently colonizing Mesoamerica, the Caribbean and North America several times. Facultative social behavior can be found in both subgenera and in most lineages, indicating that the exclusive solitary behavior found inAugochlora purais an exception. Based on morphological clues we interpret that the habit of nesting out of the soil arose once inAugochloras. str.