The knowledge acquirement by the learner is a major assignment of an E-Learning framework. Evaluation is required in order to adapt knowledge resources and task to learner ability. Assessment provides learner’s an approach to evaluate the skills gained through the e-learning domain they are accessing. A dissimilar method can be used to assess the information acquirement, such as probabilistic Bayesian Network model. A Bayesian Network is a graphical representation of the probabilistic relationships of a complex system. This network can be used for reasoning with uncertainty. Bayesian Network is the most challenging task in e-learning system as learner evaluation model are an element of uncertainty. In this paper the current proposed scheme is constructed on Bayesian Network to deduce the stage of knowledge possessed by the learner. It also proposes type of assessment to identify the knowledge whatever the learner identifies. Throughout the assessment, it can be performed by two approaches namely Sequential and Random. In Sequential approach, questions can be displayed on the learner machine in sequential order. In Random approach, questions can be displayed on the learner machine in random order. However, both have their inherent limitations. Questions that are considered to be answered easily by the learner may also be presented to the learner who is not desirable. This system determined on the illustration of Bayesian Network model and algorithm for inference about learner’s knowledge. The Bayesian Network model was efficiently implemented for three levels of learner called Higher Learners (HL), Regular Learners (RL) and Irregular Learners (IL) for learner’s assessment and was successfully implemented with 81.1% of probabilities for learner’s assessment.