Biodiesels which have been derived from non-edible vegetable oils are bagging the interest of researchers. These have been recognized as the potential alternatives of regular petroleum fuels. In this work, oil extracted from Juliflora seeds is converted as biodiesel by the transesterification process and used as alternative fuel in diesel engines. The tests are conducted by using diesel, B10, B20, B30 and B40. The experimental results of this study have disclosed that the Juliflora biodiesel blends have shown similar characteristics as diesel fuel. The BTE, CO, HC and smoke emissions are low for biodiesel blends while BSFC and NOx emissions are slightly higher. Taking all results into account it can be clinched that B10 blend of Juliflora biodiesel has viable option for diesel engine applications.