Humans have utilized natural products derived from plants as medicines for 1,000’s of years. A number of plant natural products have economic and pharmaceutical value and are essential for the scent of flowers, the flavor of fruits, and for the protection of plants against stress. Although progress has been made in the discovery of enzymes and genes in plants, the biosynthetic pathways for many natural products remain unclear. In this Research Topic of collection, we are interested in studies on the discovery, biosynthesis, regulation, transport, release, and engineering of plant natural products.