Plant derived compounds have played a vital role in the development of several chemotherapeutic agents. Catharanthus roseus is an important medicinal plant of the apocynaceae family. The leaf extract of Catharanthus roseus have many biological activities such as antibacterial, antioxidant and antidiabetes. The present study was aimed to carry out the phytochemical analysis and the GC MS analyses of Cathranthus roseus leaf extract ensure biological activity in the presence bioactive compounds. The leaves designated to the presence of secondary metabolites proteins, steroids, tannins,glycosides, reducing sugar, carbohydrates,saponins, sterols, terpenoids, acidic compounds, cardiac glycosides, phenols, alkaloids,flavonoids . In the GC MS analysis the Cathranthus roseus extract result shows the presence of bioactive compounds which revealed a broad spectrum of many medicinal property and antioxidant activity were identified. This study is helped to identify bioactive compound formula and structure which can be used as pharmaceutical industries for drug discovery. S. Nathiya | N. Shaishta Jabeen | L. Jagapriya | B. Senthilkumar | K. Devi "Estimation of Bioactive Compound of Catharanthus Roseus Leaf Extract by Phytochemical Screening and GC-MS Analysis" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-1 , December 2017, URL: https://www.ijtsrd.com/papers/ijtsrd5926.pdf