The pathogen Staphylococcus aureus is Gram positive cocci in clusters which forms golden colony on Nutrient agar. The most important characteristic feature of Staphylococcus aureus is its yellow to orange colour due to production of Staph ylox an thin. Staphyloxanthin is membrane bound Carotenoid and plays an important role in antimicrobial activity. The aim of the present study was to detect the role of Staphyloxanthin pigment production from S. aureus isolates against Staphylococcus aureus isolate from coin proving antagonistic property. The crude ethanol pigment was used against staphylococcus aureus in different concentrations ranging from 500µl to 62.5 µl by well diffusion method. Minimum inhibition was found to be 11mm at concentration range of 500. µl. The current study reported that coin plays an important role s transmitting infections Ayisha Begum | M. K. Aishwarya | Aeliya Zehra. M | Achiffa Abdul Rahiman | R. Ushasri "Staphylococcus Aureus Pigment-Bio Colour as a Novel Antibacterial Agent Against Staphylococcus Aureus Isolate from Coins" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-6 , October 2018, URL: https://www.ijtsrd.com/papers/ijtsrd15918.pdf