The sugarcane yellow mite, Oligonychus sacchari (McGregor, 1942) is one of the most important pests of sugarcane. In this study, the acaricidal efficacy of four different acaricides including Biomite®, GC-mite®, Oberon® and Envidor® was assessed under laboratory conditions at 28 ± 0.5ºC, 50 ± 5% RH and a photoperiod of 16:8 h (L:D). Adults of O. sacchari were exposed to treated leaves of sugarcane, CP57-614 variety and the mortality was counted after 24 and 48 h of exposure. Results indicated that for Biomite® the concentration of 1.5 lit/ha and in the case of GC-mite®, 1 lit/ha was more effective against O. sacchari. There were no significant differences between the Oberon® and Envidor® after 24 h of exposure. However, the toxicity of Oberon® increased after 48 h time interval. LC50 values of Biomite®, GC-mite®, Oberon® and Envidor® were 1.01, 0.86, 0.99 and 1.73 lit/ha, respectively. Application of acaricides can be effective tool for the control sugarcane yellow mite and it can be integrated with other control techniques.