Rio Grande do Sul is the southern state of Brazil and includes 569 taxa of bryophytes, a rich diversity promoted by its geographical position. All recent floristic inventories in the state recorded new occurrences of species, indicating that the diversity of bryophytes may be underestimated. Through floristic inventories carried out between 2016 and 2019, new occurrences of 16 species, included in seven families and 11 genera, were identified. Seven of them are also new records for the Southern Brazil. Bryophyta is represented by five species, four genera and four families, and Marchantiophyta by 11 species, seven genera, and three families.