The presence of Puma, Puma concolor, has been controversial in El Salvador due to the lack of published, verifiable data. We surveyed 119 sites in Montecristo National Park and 17 sites in the Río Sapo basin using wildlife cameras. We detected Pumas in both areas, representing the first photographic records for El Salvador. We call for a national Puma conservation strategy with research in basic ecology and migration corridors, regulation of hunting, management of livestock losses, and public acceptance programs. The Río Sapo basin should be granted formal protection.