We present a list of species of spiders collected at the Pico da Neblina, the highest mountain in Brazil (Amazonas, Brazil). We sampled at six altitudes (100, 400, 860, 1,550, 2,000 and 2,400 m a.s.l.), through manual active search, during the night and with a beating tray, during the day. We obtained a total of 3,143 adult individuals, which were assigned to 529 species, from 39 families. The most species rich families were Theridiidae (108 species), Araneidae (98 species) and Salticidae (60 species). Most species were rarely collected, as 389 (73% of total richness) species were represented by up to five individuals, and 197 (37% of total richness) of them by just one individual. We briefly compare our results with those from other spider surveys in the Amazon basin.