Verbascum salicifolium is described and illustrated as a species new to science based on morphological and molecular evidence. Analysis of chloroplast genome markers indicated the inclusion of the new species within a group of morphologically dissimilar taxa, whereas the closest morphologically related species is sister to that group. Additionally, the chromosome number (2n = 36) and karyotype microphotograph of the new species are provided. The known distribution of V. salicifolium is confined to the eastern shores of Doiran Lake, where a single, threatened population exists. Thus, the appropriate conservation status for the species is recommended according to IUCN criteria.