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A primer to directed evolution: current methodologies and future directions

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 01 Jan 2023 United Kingdom English Publisher:Royal Society of Chemistry (RSC)Journal:RSC Chemical Biology, volume 4, pages 271-291 (eissn: 2633-0679, Copyright policy )Funded by:UKRI | ENGINEERING SYNTHETIC MIC..., UKRI | 19-ERACoBioTech- 33 SyCoL..., EC | PERFECOAT +2 projects
Authors: Lara Sellés Vidal; Mark Isalan; John T. Heap; Rodrigo Ledesma-Amaro;

doi: 10.1039/d2cb00231k

pmid: 37034405

pmc: PMC10074555

handle: 10044/1/102800

A primer to directed evolution: current methodologies and future directions

Abstract

This review summarises the methods available for directed evolution, including mutagenesis and variant selection techniques. The advantages and disadvantages of each technique are presented, and future challenges in the field are discussed.

Country
United Kingdom
Related Organizations
Keywords

570, Chemistry, 530

  • BIP!
    Impact byBIP!
    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 46
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Top 1%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Top 10%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Top 1%
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
46
Top 1%
Top 10%
Top 1%
Green
gold