Abstract Diel vertical distribution of strictly pelagic juvenile (23–47 mm total length) maraena whitefish Coregonus maraena (Bloch, 1779) was repeatedly investigated in spring primarily using hydroacoustics in the artificial post-mining Most Lake in the Czech Republic. At the same time, an ichthyoplankton trawl was used to identify acoustical targets. During the day, fish performed extensive shoaling behaviour in depths between 2 and approximately 40 m and were not accessible for trawling. By evening, with decreasing light intensity, shoals started to disintegrate and at night fish were relatively homogeneously distributed in the water column from the surface down to a depth of 40 m. Juvenile maraena whitefish could be caught by trawl as the only fish species at night. Shoaling behaviour started again approximately 1.5 h before sunrise. The data showed steep decreases in fish density between the two surveys in spring which indicates significant mortality of early juvenile coregonids as a result of poor availability of zooplankton in a highly oligotrophic post-mining lake.